Abstract: The nano-scratch response of the nickel/copper bilayer film was studied with molecular dynamics simulation. The scratch response of the nano-nickel/copper bilayer film under different substrate textures was analyzed. The effects of tip radius and scratch depth on the scratch response of the nano-nickel/copper bilayer film were also investigated. The results showed that the extent of dislocation, due to different substrate textures, resulted in different chip sizes in front of the scratch tip. The smooth substrate surface brought to the largest chip volume. For two typical substrate textures, the scratch forces increased with the increase of the tip radius or the scratch depth when another kept constant. Moreover, when the tip radius or the scratch depth reached certain level, the substrate surface with groove texture showed certain anti-friction effect.