Abstract: In this study, a graphene oxide(GO) containing biomimic fluid was prepared for the lubrication of artificial joint, considering the poor lubrication condition and premature failure of artificial joint. A nanoscale hybrid material (GO-HA) was used as an additive with good lubricating effect of GO and good biocompatibility of hyaluronic acid (HA), which was characterized by ¹H-NMR, FTIR, TGA, AFM. The modified GO with HA was found very stable in various medium compared with naked GO. A serial of fluids were formulated by adding different amount of GO-HA into HA solutions. The performance of the lubrication fluids were examined on a tribo-meter using a ZrO₂ ball-on-plate model. The results show that the HA-GO containing fluid had obvious friction reducing and antiwear properties compared with HA solution. MTT experiment also confirmed the good biocompatibility of the fluid.